Fig. 7.

Induction of ethylene regulated gene expression in Arabidopsis expressing Zmetr2b or Zmers1b. Northern analysis was performed on total RNA from light-grown T:Zmetr2b line L9 and T:Zmers1b line L11 plants that were treated either with 100 ppm ethylene (E) or air (A) for 24 h. Following resolution and transfer of the RNA to membrane, the membrane was probed for the presence of Zmetr2b or Zmers1b mRNA using a combination of both probes (top panel). Northern analysis was also performed for chiB (basic chitinase) or PDF1.2 (plant defensin 1.2) mRNAs which are ethylene regulated. Northern analysis was also performed for the translation elongation factor 1A (eEF1A) which served as an RNA loading control. Wild-type (WT) plants were included as an ethylene sensitive control. ein2-5 and etr1-1 were included as ethylene insensitive controls