Table 3.

Effects of agl gene deletions on flagellin glycosylation in M. maripaludis.

Gene deletion	Function	Glycan structure	MW shift of flagellin	Flagella	Effect on motility
aglL	4th GTase	Missing 4th sugar and Thr on 3rd sugar	Yes	Yes	Decreased compared to WT
aglA	3rd GTase	Missing 3rd and 4th sugars	Yes	Yes	Decreased compared to aglL mutant
aglO	2nd GTase	Missing 2nd, 3rd, and 4th sugars	Yes	No	Nonmotile
aglB	OTase	No glycan	Yes	No	Nonmotile
MMP0350	Acetyl transferase	Missing 2nd, 3rd, and 4th sugars	Yes	No	Nonmotile
MMP1081	Acetamidino transfer	Missing 4th sugar and acetamidino group of 3rd sugar	Yes	Yes	Decreased compared to WT
MMP1085	Methyl transferase	Missing methyl group of 4th sugar	Yes	Yes	To be determined