Table 4.

Effects of agl gene deletions on S layer glycosylation in H. volcanii.

Gene deletion	Function	Glycan structure	Growth in high salt	MW shift of S layer	Susceptibility of S layer to proteases	Shedding of S layer	S layer symmetry
aglB	OTase	No glycan	Deficient	Yes	Same as WT	Increased	No effect
aglD	5th GTase	Missing 5th sugar	Deficient	Yes	Less than WT	Decreased	Symmetry defect
aglE	4th GTase	Missing 4th and 5th sugars	No effect	No	Same as WT	Same as WT	No effect
aglF	Glucose-1-P Uridyltransferase (sugar 3)	Mix of mono and disaccharides	No effect	Yes	More than WT	Same as WT	No effect
aglG	2nd GTase	Missing last 4 sugars	No effect	Yes	More than WT	Same as WT	No effect
aglI	3rd GTase	Mix of mono and disaccharides	No effect	Yes	More than WT	Same as WT	No effect
aglM	UDP-Glucose dehydrogenase (sugars 2 and 3)	Missing last 4 sugars	Not reported	Yes	More than WT	Not reported	Not reported
aglP	Methyl transferase (sugar 4)	Missing 5th sugar and methyl group of sugar 4	Not reported	Not reported	More than WT	Not reported	Not reported