Table 1.

Sequence of primers used for these pathogens detection by PCR analysis.

Pathogen	Primer sequence
Porcine reproductive and respiratory syndrome virus (PRRSV)	Forward primer: 5′-CCC GGG TTG AAA AGC CTC GTG T-3′
	Reverse primer: 5′- TGT AAC TTA TCC TCC CTG AAT CTG-3′
Pseudorabies virus (PRV)	gD, forward primer: 5′-CAC GGA AGA GAT GGG GCT-3′
	Reverse primer: 5′-GTC GAC GCC CGC TTG AAG CT-3′
Classical swine fever virus (CSFV)	Forward primer: 5′-TTA AAR ATA GCC CCA AAA GAG CAT G-3′
	Reverse primer: 5′-CTG GCG TCC ATC ATY CCG YGT AT-3′
Porcine parvovirus (PPV)	VP2, forward primer: 5′-GCA GTA CCA ATT CAT CTT CT-3′
	Reverse primer: 5′-TGG TCT CCT TCT GTG GTA GG-3′
Cytomegalovirus (CMV)	gB, forward primer: 5′-CCC TGA TCT TAA ATG ACG AGG ACG TGA C-3′
	Reverse primer: 5′-ACC GTC TGA GAG AGA CTG AAC TTC TCT GAC AC-3′
Porcine enterovirus (PEV)	Talfan-3D, forward primer: 5′-CAA AGA CTG GTC CTT CAT TG-3′
	Reverse primer: 5′-ATA CGC CGA GCG CGG AAG AT-3′
Porcine circovirus type 1 (PCV1)	Forward primer: 5′-ATA CGG TAG TAT TGG AAA GGT AGG G-3′
	Reverse primer: 5′-ACA CTC GAT AAG TAT GTG GCC TTC T-3′
Mycoplasma hyopneumoniae	Forward primer: 5′-GAG CCT TCA AGC TTC ACC AAG A-3′
	Reverse primer: 5′-TGT GTT AGT GAC TTT TGC CAC C-3′
Porcine coronavirus	Forward primer: 5′-GCC ATT GAT TTA TGG AGA CA-3′
	Reverse primer: 5′-GTA TAA AAC CTC CTG GCT GT-3′
Swine influenza virus (SIV)	Forward primer: 5′-AGT ATA CAG CCT AAT CAG AC-3′
	Reverse primer: 5′-AGT AGA AAC AAG GGT ATT TTT C-3′
Porcine circovirus type 2 (PCV2)	ORF-2, forward primer: 5′-GGT TTG TAG CCT CAG CCA AAG C-3′
	Reverse primer: 5′-GCA CCT TCG GAT ATA CTG TCA AGG-3′