Figure 3. T cell mediated allergic respiratory inflammation is associated with an increase in the number of lung metastases following i.v. administration of B16-F10 melanoma cells.
The increase in lung metastasis was associated with a CD4+ T cell dependent allergic inflammation but occurred independent of the allergen-induced accumulation of pulmonary eosinophils. (A) Representative photographs of lungs ressected from mice following the protocol outlined in Figure 1 showed that allergen-treated mice (wild type, OVA) experience a significant elevation in the number of metastases relative to control mice (wild type, Saline). These data also show that the allergen-induced increase in the number of metastases was lost in mice depleted of CD4+ T cells (OVA + GK1.5) but nonetheless occurred in mice devoid of eosinophils (OVA + PHIL). (B) Lung metastases were assessed by gross morphological determinations using a low power (4-10x) stereo-dissecting microscope. A quantitative summary of the mean number of lung metastases (n = 7–16 mice per group). *P<0.05.