Fig. 6.
Heparin and DS concentration dependencies of kobs. The first-order inactivation rate constant (kobs), measured in the presence of 120 μM Chromozym TH, as a function of total heparin (A) or DS (B) concentration. Recombinant (■), plasma (●), and deglycosylated (○) HCII concentrations were 84, 107, and 103 nM, respectively, and thrombin concentrations ranged from 0.6 to 2.7 nM. Solid lines represent global least-squares fitting by Eq. (5), with the fitted parameters given in the text. Experiments were performed and analyzed as described under Materials and methods.