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. 2010 May 14;38(18):6219–6233. doi: 10.1093/nar/gkq372

Figure 1.

Figure 1.

N-terminal deletion mutants of RHAU. (A) Schematic representation of the 1008 amino acid RHAU protein and its N-terminal truncated mutants. The conserved ATPase/helicase motifs I–VI of the DEAD/H-box family are indicated within the helicase core region (HCR) by vertical bars. The HCR is flanked by N-terminal (NTR) and C-terminal (CTR) regions of 203 and 393 amino acid, respectively. The N-terminal Gly-rich (amino acid 10–51) and RSM (RHAU-specific motif, amino acids 54–66) domains are indicated. WT, amino acids 1–1008; ΔGly, amino acids 50–1008; ΔGly–RSM, amino acids 105–1008; ΔRSM, RHAU harbouring a deletion of amino acids 54–66; RSM-mut, RHAU with mutagenized RSM (for details about amino acid substitutions, see Figure 6A); 1–105, amino acids 1–105; DAIH, D335A ATPase deficient mutant. (B) SDS–PAGE separation and Coomassie staining of purified FLAG-tagged recombinant wild-type (WT) and mutant RHAU proteins (2 μg protein per lane). The positions and sizes (kDa) of marker proteins are indicated at the left. The filled and open arrows on the right indicate the positions of the purified proteins.