Microarray expression in different tissues. Normalized log-scale expression of various sequences printed on a custom made microarray. Yellow circles indicate newly identified miRNAs, black dots indicate miRbase miRs, and plus signs (+) indicate all other deep sequencing detected sequences. (A) Colon tumor versus colon tumor adjacent tissue from the same patient. Labeled miRNAs are known to be expressed or deregulated in colon tumors (3,35–37,54). The newly identified miRNAs, marked in yellow, are expressed in levels similar to those of known relevant miRNAs. (B) Median of two lung tumor samples versus median expression of other tumors, from the following tissues: bile duct, bladder, breast, colon, kidney, liver, lung, ovary, pancreas and prostate. One lung tumor sample is from type non-small squamous, the other is a mix of various lung tumor types. Labeled miRNAs are known to be expressed in epithelial tumors (3). A novel miRNA, MID-19667, is preferentially expressed in lung tumors. (C) Breast primary tumor versus breast metastasis to the lymph node. Labeled miRNAs are known to be high in breast cancer or differential between breast metastasis and primary tumors (38,55).