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. 2010 May 19;38(18):6018–6028. doi: 10.1093/nar/gkq417

Figure 1.

Figure 1.

Comparison of DNA binding of purified wild-type and mutant SOX9 proteins to the Col2a1 enhancer. (A) Schematic representation of the proteins used in the study: wild-type SOX9 and dimerization mutant SOX9. (H, His-tag; S, S-tag; F, FLAG-tag; D, dimerization domain; HMG, HMG box; TA, transcription activation domain). (B) SDS–PAGE analysis of purified recombinant SOX9 polypeptides. (C) Amounts of DNA–protein complexes computed using a Phosphoimager from four sets of EMSAs with a 31-bp oligonucleotide probe corresponding to the 3′-end of the 48-bp Col2a1 intron 1 enhancer and increasing amounts of wild-type (wt) or mutant SOX9 proteins.