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Evaluation of the specificity of the Q–PCR systems targeting KRAS and PSAT1. Human KRAS H2, mouse KRAS M3, human PSAT1 H5 and mouse PSAT1 M4 primer sets were tested using plasma ctDNA extracts either from BALB/C mice (A) or from healthy human individuals (B). Results of amplifications with these PCR systems were expressed as plasma ctDNA concentration (ng/ml). Dark and light histograms represent the values of ctDNA concentration assessed in duplicates.
