Table 2.

ctDNA fragmentation relative to tumor progression in xenografts

DII was calculated as the ratio of the ctDNA concentrations obtained from the amplification of a short/133 bp and a long/290 bp DNA fragment from the human ACTB gene, and a short/120 bp and a long/338 bp DNA fragment from the mouse ACTB gene. Two representative experiments are shown in upper and lower parts. Results of amplifications are expressed in terms of concentration (ng/ml of plasma) for each tested mouse with increasing tumor weight. Data represent values of ctDNA concentration assessed in duplicates. Each mouse was identified by a different number (Mo n). ND, not detected.