Table 2.
Binding parameters obtained for acetohexamide with glycated HSA by frontal analysis and using a two-site modela
| High affinity sites | Low affinity sites | |||||
|---|---|---|---|---|---|---|
| Type of column | Ka1 (× 105 M−1) | mL1 (× 10−8 mol) | Specific activity (mol/mol HSA) | Ka2 (× 103 M−1) | mL2 (× 10−8 mol) | Specific activity (mol/mol HSA) |
| HSAb | 1.3 (± 0.2) | 2.4 (± 0.1) | 1.3 (± 0.1) | 0.35 (± 0.03) | 9.3 (± 5.5) | 5.2 (± 3.1) |
| gHSA1 | 1.2 (± 0.2) | 1.7 (± 0.1) | 1.3 (± 0.2) | 1.4 (± 0.8) | 1.7 (± 0.4) | 1.3 (± 0.3) |
| gHSA2 | 2.0 (± 0.6) | 1.8 (± 0.3) | 0.80 (± 0.20) | 11 (± 3) | 2.4 (± 0.3) | 1.1 (± 0.2) |
| gHSA3 | 2.0 (± 0.3) | 1.5 (± 0.1) | 0.80 (± 0.09) | 4.1 (± 0.7) | 3.0 (± 0.1) | 1.6 (± 0.5) |
a
The values in parenthesis represent ± 1 S.D. These values were determined by using error propagation and the standard deviations of the slopes and intercepts that were obtained from best-fit lines generated according to Eqn. (3).
b
The results for normal HSA were obtained from Ref. [18] and were acquired using immobilization and chromatographic conditions identical to those used in this current study for glycated HSA.