Table 2.

The insertion kinetics of dCTP and dTTP opposite 1,2-dihydro-2-oxoadenine (2-oxodA) by Pol IV.

Substrate	Km (fmol/ mL /min)	V max  (min−1)	k cat	k cat/Km
dCTP	8.5	217.4	4.4 × 10−3	5.2 × 10−4
dTTP	25.6	42.9	8.6 × 10−4	3.4 × 10−5

Primer 3 was labeled at the 5′-terminal by polynucleotide kinase and annealed with appropriate template oligonucleotides. Primer 3/template 2 (50 fmol) in a 10-μl reaction mixture was incubated at 20°C for 60 min with purified Pol IV (50 nM) in the presence of dTTP or dCTP at various concentrations (0.02~500 μM), followed by polyacrylamide gel electrophoresis.