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. 2010 Oct 7;2010:537531. doi: 10.1155/2010/537531

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Copyright © 2010 Yugang Wu et al.

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Expression of HIF-1α, CXCR4, and VEGF mRNA in normal colon tissues and colon cancer. The mRNA expression of HIF-1α, CXCR4, and VEGF were quantified by real-time PCR in different samples, including normal colonic tissue samples (n = 6, negative control group), colon cancer with lymph node metastasis samples (n = 6, lymph node metastasis group), and colon cancer with distant metastasis samples (n = 6, distant metastasis group). Total RNA was isolated using TRIzol according to the manufacturer's instructions and reverse transcribed. mRNA expression of HIF-1α, CXCR4, VEGF and β actin was determined by real-time PCR using SYBR Green master mix (see Section 2). Differences in the relative level of three markers normalized to β actin can be estimated by differences in the ratio. CXCR4, 15.91 ± 12.14-fold in lymph node metastasis group or 23.20 ± 14.84-fold in distant metastasis group versus 6.23 ± 4.66-fold in negative control group, respectively, P < .05, VEGF, 9.99 ± 3.38-fold in lymph node metastasis group or 10.27 ± 5.90-fold in distant metastasis group versus 4.68 ± 1.28-fold in negative control, respectively, P < .05.