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. 2010 Aug 18;17(10):1507–1516. doi: 10.1128/CVI.00189-10

FIG. 3.

FIG. 3.

CD8+ T cell line IFN-γ ELISPOT responses obtained with the use of HLA A2 cells as APC. PBMC from healthy HLA A*0201-positive HHV-8-seropositive donors were stimulated with peptide, with IL-2 added every 3 days. On day 15, B cell lines expressing only HLA A*0201 (A2 cells) or no HLA molecules (null cells) were used as APC. IFN-γ production was measured by an ELISPOT assay as described for Fig. 1. Responses to novel epitopes from LANA-1, K12, gB, K8.1, and a known epitope from gB (gB492-500) (40) are shown for one representative donor out of two donors used.