Polyfunctional CD8+ T cell responses to known and novel HHV-8 epitopes. CD14− PBMC from healthy HLA A*0201-positive HHV-8-seropositive donors were stimulated for 1 week with autologous mature DC loaded with known HHV-8 epitopes (5, 6, 12, 40) (A) or novel epitopes from LANA-1 (B) or K12, gB, or K8.1 (C). Responses were measured by polychromatic flow cytometry, and net response values were averaged for three donors (mean + SE). Diagrams were generated using SPICE.