Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Purification of klassevirus 3C protease. N-terminal His-tagged klassevirus 3C protease was expressed in E. coli and purified by Ni-NTA chromatography (A and B). Ni-NTA eluates were further purified by gel filtration chromatography using a Superdex 200 column (C). The 91-ml fraction was tested via silver stain to confirm purity of klassevirus 3C protease (D). Lane M, molecular mass markers.
