FIG. 2.

Klassevirus 3C ELISA for matched serum samples from klassevirus PCR-positive children from India. Endpoint OD₄₅₀ values for matched preinfection serum samples (1:100 dilution; white) and postinfection serum samples (1:100 dilution; shaded) are grouped together, along with polyclonal rabbit antisera generated against klassevirus 3C and blocking buffer (A). The dotted line indicates an OD₄₅₀ cutoff value of 0.30 that separates pre- and postinfection OD₄₅₀ values. Asterisks indicate significant increases in OD₄₅₀ (P < 0.005). Remaining serum was available from two matched serum samples (2246 and 3516) for Western confirmation, which showed a 4-fold and 8-fold increase in signal between pre- and post-klassevirus infection serum samples at a dilution of 1:200, respectively (B).