TABLE 1.
Serologic responses to M. tuberculosis proteins in protein array and ELISA screens
| H37Rv locus tag | Gene name | Molecular mass (kDa) | Reference for previously described immune function | Functional categorya | Initial target selectionb | Protein array FOC value | ELISA antibody responsec |
|---|---|---|---|---|---|---|---|
| Rv0054 | ssb | 17.3 | 2 | S | 4 | + | |
| Rv0164 | TB18.5 | 17.7 | 10 | S | 3 | ++ | |
| Rv0410 | pnkG | 81.6 | 9 | S | 3 | + | |
| Rv0455c | Hypothetical | 16.6 | 10 | S | 1 | + | |
| Rv0655 | mkl | 39.3 | 3 | M | 4 | ++ | |
| Rv0831c | Hypothetical | 30.2 | 10 | S | 2 | +++ | |
| Rv0934 | pstS1 | 38.2 | 17 | 3 | S | 4 | +++ |
| Rv0952 | sucD | 31.2 | 7 | B | 3 | − | |
| Rv1009 | rpfB | 38 | 3 | M | 4 | + | |
| Rv1099 | Hypothetical | 34.6 | 10 | M | 3 | ++ | |
| Rv1240 | mdh | 34.3 | 7 | H | ND | + | |
| Rv1288 | Hypothetical | 49.6 | 10 | B | 3 | + | |
| Rv1410c | p55 | 54.7 | 3 | M | 3 | + | |
| Rv1569 | bioF1 | 40 | 7 | M | ND | ++ | |
| Rv1789 | PPE26 | 38.6 | 6 | P/E | 3 | ++ | |
| Rv1813c | Hypothetical | 15 | 4 | 10 | H | 3 | − |
| Rv1827 | cfp17 | 17.2 | 43 | 10 | EC | ND | + |
| Rv1837 | mtb81 | 80.7 | 14 | 10 | M | ND | ++ |
| Rv1860 | apa | 32.7 | 8 | 3 | S | 3 | + |
| Rv1886c | fpbB | 34.6 | 42 | 1 | S | 3 | +++ |
| Rv1908 | katG | 80.6 | 46 | 0 | M | ND | + |
| Rv1984c | cfp21 | 21.8 | 44 | 3 | S | 3 | −/+ |
| Rv2031 | acr | 16.2 | 20 | 0 | S | 3 | + |
| Rv2032 | acg | 36.6 | 10 | H | 5 | +++ | |
| Rv2220 | glnA1 | 53.5 | 41 | 7 | S | 1 | ++ |
| Rv2450 | rpfE | 17.4 | 3 | B | 4 | −/+ | |
| Rv2608 | PPE42 | 59.7 | 6 | 6 | P/E | 2 | +++ |
| Rv2623 | TB31.7 | 31.7 | 10 | H | 4 | + | |
| Rv2866 | Hypothetical | 10.2 | 10 | H | 7 | + | |
| Rv2873 | mpt83 | 20 | 15 | 3 | S | 3 | ++ |
| Rv2875 | mpt70 | 19.1 | 32 | 3 | S | 4 | +++ |
| Rv3020 | esxS | 9.8 | 3 | P/E | 3 | −/+ | |
| Rv3044 | fecB | 36.9 | 3 | H | 1 | + | |
| Rv3310 | sapM | 31.8 | 3 | S | ND | ++ | |
| Rv3407 | Hypothetical | 11 | 29 | 0 | B | 3 | −/+ |
| Rv3611 | Hypothetical | 23.8 | 16 | M | 3 | − | |
| Rv3614 | Hypothetical | 19.8 | 10 | M | 5 | ++ | |
| Rv3619 | esxI | 9.8 | 3 | P/E | 6 | + | |
| Rv3628 | ppa | 18.3 | 7 | S | 3 | −/+ | |
| Rv3841 | bfrB | 20.4 | 33 | 7 | EC | ND | ++ |
| Rv3874 | cfp10 | 10.8 | 10 | 3 | P/E | ND | ++ |
| Rv3881 | mtb48 | 47.6 | 24 | 10 | S | ND | ++ |
As defined by Tuberculist (http://genolist.pasteur.fr/TubercuList/index.html). 0, virulence, detoxification, adaptation; 1, lipid metabolism; 2, information pathways; 3, cell wall and cell processes; 6, PE/PPE proteins; 7, intermediary metabolism; 8, unknown; 9, regulatory proteins; 10, conserved hypothetical proteins; 16, conserved hypothetical proteins with an ortholog in M. bovis.
EC, expression cloning; S, secreted proteins; P/E: PE, PPE, and EsX proteins; M, macrophage growth required; H, hypoxic response; B, other database searches.
−, <2-fold mean difference between TB-positive and -negative controls; −/+, near mean cutoff with fewer than 12% of the TB-positive serum samples; +, ++, and +++, percentages of TB-positive responders (12 to 28%, 29 to 43%, and >44%, respectively).