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Comparison of the TcBCDE ELISA with the Biokit Chagas ELISA and the Bios Chile Chagas ELISA. The same sets of 65 sera from T. cruzi-positive patients and of 65 negative-control sera from SEDES, Cochabamba, were tested under similar conditions (5 μl of serum each for the reaction with antigen, and peroxidase-conjugated anti-human IgG as secondary antibody). Calculated cutoff values are indicated by horizontal lines.
