TABLE 1.
Synthetic tandem repeat antigens of T. cruzi
| Antigen | Amino acid sequence | No. of repeats in genome | Similarity with Leishmaniaspp. |
|---|---|---|---|
| MAP | PRHVDPDHFRSTTQDAYRPVDPSAYKRALPLEEEEDVG PRHVDPDHFRSTTQDAYRPVDPSAYKRALPLEEEEDVG PRHVDPDHFRSTTQDAYRPVDPSAYKRALPQEEEEDVG | 135 | 40% (L. infantum), 52% (L. braziliensis) |
| JL8 | AAEATKVAEAEKQR AAEATKAVEAEKQR AAEATKVAEAEKQK | 140 | Very weak similarity |
| CRA | KVAEAEKQKAAEAT KVAEAEKQKAAEAT KVAEAEKQKAAEAT | 130 | No similarity |
| B13 | PFGQAAAGDKPS PFGQAAAGDKPS PFGQAAAGDKPK | 103 | No similarity |
| FRA | AFLDQKPEGVPLRELPLDDDSDFVAMEQERRQLLEKDPRRNAREIAAL EESMNARAQELAREKKLADR AFLDQKPEGVPLRELPLDDDSDFVAME QERRQLLEKDPRRNAKEIAALEESMNARAQELAREKKLADR AFLDQK | 14 | 70% (L. infantum), 63% (L. braziliensis) |
| FRA2a | MEQERRQLLEKDPRRNAREIAALEESMNARAQELAREKKLADRAFPDSPNSMEQERRQLLEKDPRRNAREIAALEESMNARAQELAREKKLADRAFPNSPDMEQERRQLLEKDPRRNAREIAALEESMNARAQELAREKKLADRAF | 14 | 55% (with both L. infantumand L. braziliensis) |
| TcD | PKPAE PKPAE PKPAE PKPAE PKPAE PKPAE PKPAE PKPAE PKPAE | 430 | No similarity |
| TcE | PAKAAA PPAKAAA PPAKAAA PPAKAAA PPAKAAA PPAKAAAP | 70 | No similarity |
| SAPA | PVDSSAHGTPST PVDSSAHGTPST PVDSSAHSTPST PVDSSAHSTPST PADSSAHSTPST | >130 | No similarity |
| TcMyo | LAQREADNEKLAED LAQREADNEKLAEE LAQREADNEKLTED LAQREADNEKLAED | >170 | No similarity |
a
Underlined residues indicate introduced proline-rich spacer sequences.