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. 2010 Aug 4;17(10):1517–1523. doi: 10.1128/CVI.00108-10

FIG. 3.

FIG. 3.

Anti-EV71 immune serum can enhance EV71 infection on THP-1 cells at subneutralizing concentrations. THP-1 cells were infected with EV71 4643 stain at an MOI of 10 for 24 h with or without the presence of immune serum. The EV71 infection was detected by immunofluorescent microscopy or determined by flow cytometry at 24 h postinfection. (A) Immunofluorescence staining of EV71 VP1+ on THP-1 cells by immune serum at a 10−3 dilution. (B) Flow-cytometric analysis of EV71 VP1+ on THP-1 cells by immune serum at a 10−3 dilution. (C) The dose range of enhancement of EV71 infection on THP-1 cells by anti-EV71 immune serum. The sera were obtained from EV71-infected patients and diluted to various concentrations. The dilution at 10−3 shows the highest enhancement. Coxsackievirus A16 (CA16) immune serum was collected from CA16-infected patients and used as an antibody specificity control. The neutralization titer of immune serum to EV71 is 512.