FIG. 3.

Scouring effects of cutinase-CBMs and cutinase on cotton fiber. (A) Adsorption on cotton fiber. Purified cutinase or cutinase-CBM fusion protein was incubated in 20 mM Tris-HCl buffer (pH 8.0, 25°C). (B) Analysis of products released from cotton fiber. A 50 μM concentration of purified cutinase or cutinase-CBM fusion protein was incubated in 20 mM Tris-HCl buffer (pH 8.0, 50°C) with or without pectinase, and the amount of released fatty acids was measured by titration with 0.02 N NaOH.