TABLE 1.
Primers used in the construction of the fusion genes
| Primer | Sequence (5′-3′)a | Restriction enzyme |
|---|---|---|
| P1 | GGAATACCATATGTCCATGGCCAACCCCTACGAGCGCGG | NcoI |
| P2 | CGCGGCGATCGCCATGAACGGGCAGGTGGA | |
| P3 | TCCACCTGCCCGTTCATGGCGATCGCCGCG | |
| P4 | CATCTCGAGAGAATTCGGGCAGGTAAGGGTCGGAACAG | EcoRI |
| P2′ | GCGGCAGCCGGGAGCGGGAGGCGGCGTGGG | |
| P3′ | CCCACGCCGCCTCCCGCTCCCGGCTGCCGC | |
| P4′ | CATCTCGAGAGAATTCGGGGTGCCCGTGCAGGTGGTGC | EcoRI |
a
Restriction enzyme sites are indicated in boldface. Portions (15 bp) of the overlapping regions are underlined.