TABLE 2.

Monomeric products released from cutin hydrolysis by cutinase-CBMs and cutinase^a

Cutin hydrolysis product	Hydrolysis area (%)
	Cutinase	Cutinase-CBMCel6A	Cutinase-CBMCenA
Hexadecanoic acid	27.6	30.6	31.4
Octadecenoic acid	26.7	25.2	27.5
9-Octadecenoic acid	0.50	0.43	0.36
9,12-Octadecadienoic acid	1.08	1.02	1.01
16-Hydroxyhexadecanoic acid	0.54	0.54	0.58
18-Hydroxyoctadeca-9-enoic acid	1.01	0.87	0.91
18-Hydroxyoctadeca-9,12-dienoic acid	1.12	1.76	1.70
9,10,18-Trihydroxyoctadecanoic acid	0.47	0.47	0.51

^aCutin hydrolysis by cutinase-CBM and cutinase was carried out in 25 mM potassium phosphate buffer (pH 8.0) at 50°C, for 18 h. Each value represents the mean of three independent measurements, and the variation about the mean is below 5%.