TABLE 2.
Target sequences, cycling conditions, and oligonucleotide primers
| Target organism | Target gene; GenBank no. | Primer annealing temp (°C); primer extension time (s) | Primer names (reference) |
|---|---|---|---|
| E. coli | Glucuronidase; S69414 | 60; 15 | Eco-F, Eco-R (23) |
| E. faecalis | 23S rRNA gene; AE016830 | 58; 15 | ECF, ECR (10) |
| C. perfringens | Alpha-toxin; AY277724 | 60; 15 | cpaF, cpaR (8) |
| EHEC and EPECa | Intimin; AF081182 | 55; 15 | EAE-a, EAE-b (7) |
| C. jejuni | VS1; X71603 | 55; 20 | forward, reverse (24) |
| L. monocytogenes | Listeriolysin; M24199 | 60; 15 | forward, reverse (19) |
| S. enterica | Invasin; U43272 | 68; 15 | invA139, invA141 (7) |
| Y. pseudotuberculosis | Invasin; M17448 | 62; 15 | inv-F, inv-R (25) |
| C. burnetii | com1; AF318146 | 60; 15 | FAF216, RAF290 (1) |
| L. interrogans | Lipoprotein L32; AF181553 | 60; 30 | 270F, 692R (16) |
| M. avium subsp. paratuberculosis | IS900; X16293 | 60; 15 | F2, R2 (3) |
| Cryptosporidium spp. | COWPb; AF248743 | 60; 15 | P702F, P702R (9) |
| Giardia spp. | β-Giardin; M36728 | 60; 15 | P241F, P241R (9) |
a
EHEC, enterohemorrhagic E. coli; EPEC, enteropathogenic E. coli.
b
COWP, cryptosporidium oocyst wall protein.