TABLE 4.
Frequency of DNA detection by qPCR of zoonotic pathogens in manures of Australian feedlot cattle
| Target organism | % detection (no. of analyses)b |
|||||
|---|---|---|---|---|---|---|
| Fresh feces | Pen manure | Harvested manure | Aged manure | Composted manure | Carcass compost | |
| Pathogenic E. colia | 81 (32) | 69 (32) | 32 (25) | 20 (20) | 17 (6) | 14 (7) |
| C. jejuni | 94 (32) | 38 (32) | 0 (25) | 5 (20) | 0 (6) | 0 (7) |
| L. monocytogenes | 31 (32) | 34 (32) | 16 (25) | 35 (20) | 0 (6) | 43 (7) |
| S. enterica | 6 (32) | 0 (32) | 0 (25) | 0 (20) | 0 (6) | 0 (7) |
| Y. pseudotuberculosis | 3 (32) | 6 (32) | 4 (25) | 5 (20) | 0 (6) | 0 (7) |
| C. burnetii | 3 (32) | 0 (32) | 4 (25) | 0 (20) | 0 (6) | 0 (7) |
| Leptospira spp. | 0 (32) | 3 (32) | 0 (25) | 0 (20) | 0 (6) | 0 (7) |
| M. avium subsp. paratuberculosis | 0 (32) | 0 (32) | 8 (25) | 0 (20) | 0 (6) | 0 (7) |
| Cryptosporidium spp. | 13 (32) | 16 (32) | 8 (25) | 15 (20) | 0 (6) | 0 (7) |
| Giardia spp. | 34 (32) | 0 (32) | 8 (25) | 30 (20) | 0 (6) | 43 (7) |
a
Positive for virulence marker gene eaeA.
b
Data for assays under the detection limit are shown in boldface.