FIG. 3.
Kinetics of Hg adsorption to PHL628 strains at pH 7.2. Suspended cultures of PHL628 and PHL628 csgA were grown in GMM at 30°C to stationary phase. Aliquots of concentrated Hg solution were added to the cultures to yield total concentrations of 0.25 μM and 0.75 μM Hg. Aliquots were withdrawn at predetermined intervals, and soluble Hg concentrations were determined. Adsorbed Hg normalized by biomass was calculated as the difference between the total added and dissolved Hg concentrations, and after correcting for background values, it was divided by the OD600 and plotted versus time. The points and whisker bars indicate the average and the range of the results for duplicate samples; the gray lines represent the calculated fit.