TABLE 2.
Production of A54145 factors in S. ambofaciens BES2074 and S. roseosporus UA431
| Strain | Plasmid | Lipopeptide yield (mg/liter) | % of controla | A-core (%) |
|---|---|---|---|---|
| S. fradiae NRRL 18160 | 423 ± 11 | 100 | 63 | |
| S. ambofaciens DA1163 | pDA2002 | 362 | 86 | 22 |
| S. ambofaciens DA1164 | pDA2002 | 410 | 97 | 27 |
| S. ambofaciens DA1168 | pV89 | 0 | 0 | 0 |
| S. roseosporus DA1155 | pDA2002 | 106 | 25 | 22 |
| S. roseosporus DA1156 | pDA2002 | 95 | 22 | 2 |
| S. roseosporus DA1160 | pV89 | 0 | 0 | 0 |
Results for the control, S. fradiae NRRL18160, are shown here and in Table 3. The lipopeptide yields from heterologous expression strains were determined from single fermentations in a separate experiment.