TABLE 4.

Production of lipopeptides by mutants defective in lptI methyltransferase and results of trans-complementation with lptI and dptI genes

Strain	Deletion	Complementation vector	Gene(s) on vector	Lipopeptide yield (mg/liter)	% of controlb	A-core (%)
XH25				472 ± 29	100	62
DH613	ΔlptI::tsr			677 ± 24	141	100
JR397	ΔlptI			692 ± 41	138	100
DA1096	ΔlptI::tsr	pCV16	lptIa	550 ± 18	115	76
DA1087	ΔlptI::tsr	pDA1658	ermE*p-lptI	556 ± 16	116	83
DA1077	ΔlptI::tsr	pDA1660	ermE*p-dptI	413 ± 40	86	15

^alptI was transcribed from its native promoter. Cosmid pCV16 also has the lptDGHJKLMN genes split from the promoter upstream of lptEF, lptP, and other genes not involved in A54145 biosynthesis downstream of lptI (36).

^bCompared to results with S. fradiae XH25 (control).