TABLE 5.

Lipopeptide production by ectopically trans-complemented lpt deletion mutants^b

Strain	Deletion(s)	Insertion via φC31	Insertion via φBT1	Gene changea	Yield (mg/liter)	% of controlb	A-core (%)
XH25					472 ± 29	100	62
DA1676	ΔlptEF-I	lptEF-I			513 ± 16	109	60
DA1324	ΔlptEF-I		lptEF-I		583 ± 95	124	61
DA1674	ΔlptEF-I	lptEF-P		ΔlptI	524 ± 19	112	100
DA1204	ΔlptBCD ΔlptI	lptB-I		lptG-P	395 ± 11	84	52
DA1206	ΔlptBCD ΔlptI	lptB-H		ΔlptI, lptGH	419 ± 39	89	100
DA1444	ΔlptBCD ΔlptI	lptBC	lptD-I	lptG-I	408 ± 48	87	36
DA1102	ΔlptD	lptD-I		lptG-I	478 ± 26	101	32
DA1106	ΔlptD	lptB-I		lptBC, lptG-I	486 ± 80	103	38
DA1109	ΔlptBCD	lptB-I		lptG-I	444 ± 18	94	20

^alpt genes missing (Δ) or duplicated (shown in bold) by the complementing plasmid are listed.

^blptEF-I, lptEFABCDGHJKLMNPI; lptEF-P, lptEFABCDGHJKLMNP; lptB-I, lptBCDGHJKLMNPI; lptG-P, lptGHJKLMNP; lptB-H, lptBCDGH; lptD-I, lptDGHJKLMNPI; lptG-I, lptGHJKLMNPI.