TABLE 2.
Primers used for qPCR experiments
| cDNA | Gene reference no. | Function | Primer sequence | Fragment size (bp) |
|---|---|---|---|---|
| UBI | CV543388a | Ubiquitin | GAGGATGGTCGCACCCTGGCT | 184 |
| CCCTCCTTGTCCTGAATCTTA | ||||
| EF1-α | GI151368189b | Elongation factor 1α | CAAGGATCTCAAGCGTGGTTTCG | 150 |
| TGGGAGGTGTGGCAATCAAGC | ||||
| JAR1 | GI171655005b | Jasmonate-amino synthetase | AAGAACCGGGACACTATGTGA | 174 |
| AATGTTCCCCTCCGAACAAC | ||||
| PR3 | GI62703651b | PR3 protein (chitinase) | TAGGAGTGGTGTGGATGCTG | 192 |
| CGCTGAGATCAGTAGGAGCA | ||||
| ACC | CV541144a | ACC oxidase | TGGCACCAAAGTTACAACTA | 270 |
| ATTCTGGTGCCATCCGTTTGA |
a
cDNA library of Phaseolus vulgaris (22).
b
Reference numbers in NCBI, Phaseolus vulgaris cDNA.