FIG. 2.

Culture growth and detection of proteolytic activities with chromogenic substrates. Growth of the culture growth was monitored by measuring the optical density at 600 nm (OD₆₀₀) (filled symbols • and ▪ for X. kozodoii Morocco and Intermedium strains, respectively). The data shown are representative of four measurements for both cultures. Enzyme activities were measured with Fua-LGPA and Fua-ALVY substrates (open symbols ⋄ and ▵, respectively). The data are representative of two measurements on both substrates in the culture of X. kozodoii Morocco strain. The activities were measured using 50 μl of culture supernatant at a final concentration of substrate of 50 μM.