TABLE 3.
Effects of inhibitors on the observed protease activities
| Inhibitor | Remaining activitya on: |
||
|---|---|---|---|
| Succ-AAPF-Sbzl | Fua-LGPA | Fua-ALVY | |
| EDTA | 99.1 ± 11.2 | 1.3 ± 2.2 | 0.1 ± 0.1 |
| 1,10-Phenanthroline | 105.3 ± 15.1 | 0.1 ± 1.5 | 0.9 ± 0.5 |
| PMSF | 14.8 ± 7.5 | 86.3 ± 5.4 | 16.6 ± 6.2 |
| Cys | NT | 29.7 ± 11.0 | 0.2 ± 0.3 |
| Thimerosal | NT | 97.0 ± 5.2 | 56.3 ± 8.8 |
| DTT | NT | 68.5 ± 7.8 | 0.5 ± 0.2 |
a
Activity expressed as a percentage of the activity of the control. The results are from measurements on two different culture supernatants, and the data shown are the means ± standard errors of the means of at least three measurements. The control (100%) values for the Succ-AAPF-SBzl-ase, Fua-LGPA-ase, and Fua-ALVY-ase activities are (2.90 ± 0.21) × 10−7 M/min, (7.6 ± 0.5) × 10−7 M/min, and (3.4 ± 0.42) × 10−6 M/min, respectively. NT, not tested.