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. 2010 Aug 27;76(20):6901–6909. doi: 10.1128/AEM.01567-10

TABLE 3.

Effects of inhibitors on the observed protease activities

Inhibitor Remaining activitya on:
Succ-AAPF-Sbzl Fua-LGPA Fua-ALVY
EDTA 99.1 ± 11.2 1.3 ± 2.2 0.1 ± 0.1
1,10-Phenanthroline 105.3 ± 15.1 0.1 ± 1.5 0.9 ± 0.5
PMSF 14.8 ± 7.5 86.3 ± 5.4 16.6 ± 6.2
Cys NT 29.7 ± 11.0 0.2 ± 0.3
Thimerosal NT 97.0 ± 5.2 56.3 ± 8.8
DTT NT 68.5 ± 7.8 0.5 ± 0.2
a

Activity expressed as a percentage of the activity of the control. The results are from measurements on two different culture supernatants, and the data shown are the means ± standard errors of the means of at least three measurements. The control (100%) values for the Succ-AAPF-SBzl-ase, Fua-LGPA-ase, and Fua-ALVY-ase activities are (2.90 ± 0.21) × 10−7 M/min, (7.6 ± 0.5) × 10−7 M/min, and (3.4 ± 0.42) × 10−6 M/min, respectively. NT, not tested.