FIG. 3.

In vivo ³¹P-NMR spectrum (A) and measurements to determine the cytoplasmic concentrations of intracellular P_i, phosphate monoesters (PME), NDP-glucose, and polyphosphate (polyP) in cells cultivated for 8 h (a) and 24 h (b) in CGXII glucose minimal medium with 13 mM P_i (black bars) or for 8 h (c) and 24 h (d) in CGXII glucose minimal medium with 0.13 mM P_i (white bars) (B). Signals representing intracellular P_i (1), phosphate monoesters (2), NDP-glucose (3), and polyphosphate (4) are marked in the ³¹P-NMR spectra, which were recorded using a Varian Inova 400 MHz spectrometer operating at a ³¹P frequency of 161.985 MHz, as described in Materials and Methods. The experiment was performed twice with comparable results.