TABLE 3.
Influence of Pi concentration on the expression of the pgm and glgC genes in C. glutamicum
| Growth condition | CAT activitya (nmol min−1 mg protein−1) |
|
|---|---|---|
| C. glutamicum pET2-pgm | C. glutamicum pET2-glgC | |
| Pi excess (13 mM) | 31 ± 5 | 195 ± 37 |
| Pi limitation (0.13 mM) | 189 ± 37 | 583 ± 6 |
a
Strains were cultivated for 24 h in CGXII medium with 222 mM glucose under either Pi excess or Pi limitation. Chloramphenicol acetyltransferase activities were determined using cell-free extracts. Means and standard deviations derived from three independent cultivations are given.