FIG. 10.

p27^Kip1 regulates the proliferative renewal of memory CD8 T cells. At day 80 p.i., T cells were purified from the spleens of +/+ and −/− mice, labeled with CFSE, and adoptively transferred into naïve congenic B6/Ly5.1 mice. Four weeks after cell transfer, CFSE dilution in donor LCMV-specific CD8 T cells was assessed by flow cytometry. (A) Proliferative renewal of NP396- and GP33-specific CD8 T cells. The histograms are gated on D^b/NP396 or D^b/GP33 tetramer-binding Ly5.2-positive CD8 T cells. (B) Proliferative renewal of central memory (CM) and effector memory (EM) CD8 T cells. The histograms are gated on D^b/NP396 tetramer-binding Ly5.2-positive CD8 T cells. Effector memory (CD62L^low) and central memory (CD62L^high) subsets were identified based on cell surface expression of CD62L.