FIG. 6.

Preferential expansion and maintenance of KLRG-1^low IL-7Rα^high MPEC CD8 T cells in p27^Kip1-deficient mice. (A and B) At the indicated number of days after LCMV infection, splenocytes were stained with anti-CD8, anti-KLRG-1, anti-IL-7Rα, and D^b/NP396 tetramers (A) or D^b/GP33 tetramers (B). The absolute numbers of KLRG-1^low IL-7Rα^high (MPECs) and KLRG-1^high IL-7Rα^low (SLECs) NP396 or GP33 tetramer-binding CD8 T cells were calculated. Data are representative of three to seven independent experiments for each time point. (C) Proliferation of MPECs and SLECs in −/− mice. LCMV-infected +/+ and −/− mice were treated with BrdU between days 8 and 15 p.i., and the percentages of NP396- or GP33-specific BrdU-positive MPECs and SLECs were determined at day 15 p.i. The histograms are gated on NP396- or GP33-specific MPECs or SLECs, and the numbers are the percentages of BrdU-positive cells among the gated cell population; data are from three mice/group.