FIG. 9.
p27Kip1 regulates the quantity and quality of CD8 T-cell memory. Wild-type and −/− mice were infected with LCMV. (A and B) At day 112 p.i., splenocytes were stimulated with LCMV epitope peptides, and the percentages of IFN-γ-, TNF-α-, and IL-2-producing CD8 T cells were assessed by intracellular cytokine staining. (A) Total number of epitope-specific IFN-γ-producing CD8 T cells in +/+ and −/− mice. (B) Antigen-induced cytokine production by LCMV-specific CD8 T cells. The data are percentages of epitope-specific CD8 T cells producing IFN-γ, TNF-α, and IL-2. (C) At day 78 p.i., the cell surface phenotype of virus-specific CD8 T cells was assessed by flow cytometry. Plots are gated on tetramer-binding CD8 T cells. The numbers are the percentages among tetramer-binding CD8 T cells, or the mean fluorescent intensity of staining for the indicated marker.