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Primary adipocyte analysis demonstrates Akt-independent and PI3K-dependent regulation of lipolysis by insulin. Primary rat adipocytes were isolated and a glycerol release assay was performed using the following conditions: isoproterenol (Iso; 10 nM), insulin (Ins; 20 nM), Akt inhibitor (Akti; 10 μM), and wortmannin (W/M; 100 nM). *, P < 0.05 versus basal values.
