FIG. 8.

Lack of correlation between total cellular PKA activity and glycerol release. Duplicate sets of cells were assessed in parallel, one for glycerol release and another for kinase activity under the indicated conditions: isoproterenol (Iso; 6 nM), insulin (Ins; 25 nM), Akt inhibitor (Akti; 10 μM), and wortmannin (W/M; 100 nM) for 5 min. PKA activity was assessed from lysates incubated with and without Kemptide in the presence and absence of PKI. Data are expressed as means ± standard errors of the means from three experiments. *, P < 0.05 versus isoproterenol alone. ns, no significance versus isoproterenol alone.