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. 2010 Aug 23;30(21):4980–4995. doi: 10.1128/MCB.00004-10

FIG. 7.

FIG. 7.

Role of the lipid phosphatase/protein phosphatase activity of PTEN in the regulation of Src/Stat3 function and podosome formation. (a to f) SMC-SrcY527F cells were transiently transfected with various PTEN expression constructs either alone (a and b) or in combination with a GFP expression vector (pBabe-GFP) (c to f). Cells overexpressing either wt PTEN (a) or PTEN-G129E (b) form fewer podosomes than nontransfected cells. Fluorescent microscopic images show the effect of overexpressed PTEN-G129E (c and e) or PTEN-C124S (d and f) on Stat3 and caldesmon (Cald) expression. Bars, 20 μm. (g) SMC-SrcY527F cells were transiently transfected with either an empty vector (control) or various PTEN expression constructs (8 μg DNA per 10-cm-diameter plate) using the Lipofectamine Plus protocol. The cell lysates were prepared 48 h posttransfection and were Western blotted for proteins/phosphoproteins as shown. GAPDH protein levels were used as a loading control. (h) SMC-SrcY527F cells were transfected with pBabe-GFP either alone (control) or along with wild-type/mutant PTEN expression constructs as indicated and were assayed for podosome/rosette formation. Error bars represent standard deviations of three independent measurements. Asterisks indicate significant differences (P < 0.05) from control values.