FIG. 2.

Subcellular fractionation of mouse liver cells. (A) The contents of fresh mouse liver cells were fractionated by the detergent-based method (see Materials and Methods). The numbers refer to the fraction numbers. The distribution of RNase H1 was analyzed by gel renaturation assay and is presented in a negative image. Distributions of GAPDH, COX IV, Tom20, and fibrillarin were analyzed by immunoblotting. (B) Intact mitochondria were isolated from mouse liver and incubated with proteinase K and/or Triton X-100. RNase H1 was detected by gel renaturation assay and is presented in a negative image. Tom20 was detected by immunoblotting.