FIG. 5.
Fli1+ and Fli1ΔCTA cooperate with GATA-1 to activate transcription of the c-mpl promoter in vitro but show differential association with promoters in vivo. (A) HeLa cells were transfected with the indicated transcription factors (200 ng) and a c-mpl-luciferase reporter plasmid (200 ng). Relative activation of the reporter is shown. (B) Western blot analysis using polyclonal anti-Fli1 or anti-GATA-1 antibody on the same cell extracts. (C) Real-time PCR analysis of ChIP products. Chromatin was prepared from wild-type or Fli1ΔCTA/Fli1ΔCTA MKs, and chromatin immunoprecipitation assays were performed using anti-Fli1 or anti-GATA-1 antibodies or IgG (control). “Input” represented 5% total cross-linked, reversed chromatin before immunoprecipitation. Values represent percentages of input.