TABLE 2.
Primers used for PCR and DNA sequencing
| Locus | Gene product | Length of sequence obtained (bp) | Primer |
Useb |
Reference(s) or source | ||
|---|---|---|---|---|---|---|---|
| Designation | Sequence (5′-3′)a | PCR | Sequencing | ||||
| EF-1α | Translation elongation | 639-683c | EF1 | ATGGGTAAGGARGACAAGAC | • | 52 | |
| factor 1α | EF2 | GGARGTACCAGTSATCATG | • | 52 | |||
| EF3 | GTAAGGAGGASAAGACTCACC | • | 56 | ||||
| EF22T | AGGAACCCTTACCGAGCTC | • | 52 | ||||
| RPB1 | RNA polymerase largest | 1,607 | Fa | CAYAARGARTCYATGATGGGWCd | • | 29 | |
| subunit | G2R | GTCATYTGDGTDGCDGGYTCDCC | • | Benjamin Hall, unpublished datae | |||
| R8 | CAATGAGACCTTCTCGACCAGC | • | This study | ||||
| F5 | ATGGGTATYGTCCAGGAYTC | • | This study | ||||
| F6 | CTGCTGGTGGTATCATTCACG | • | This study | ||||
| F7 | CRACACAGAAGAGTTTGAAGG | • | This study | ||||
| F8 | TTCTTCCACGCCATGGCTGGTCG | • | This study | ||||
| R9 | TCARGCCCATGCGAGAGTTGTC | • | • | This study | |||
| RPB2 | RNA polymerase second | 1,700-1,742f | 5f2 | GGGGWGAYCAGAAGAAGGC | • | • | 68 |
| largest subunit | 7cr | CCCATRGCTTGYTTRCCCAT | • | • | 39 | ||
| 7cf | ATGGGYAARCAAGCYATGGG | • | • | 39 | |||
| 11ar | GCRTGGATCTTRTCRTCSACC | • | • | 39 | |||
a
D = A, G or T; R = A or G; S = C or G; W = A or T; Y = C or T.
b
•, primer was used for indicated purpose. RPB1 PCR primer G2R was designed by Benjamin Hall (http://faculty.washington.edu/benhall/).
c
The partial EF-1α sequence size range.
d
Fa is identical to RPB1-DF2asc (29), except that the 3′-most nucleotide was deleted.
f
The size difference is due to 3-bp and 39-bp inserts in 5 × 7 RPB2 fragment in members of the Fusarium dimerum species complex and the Gibberella clade, respectively.