TABLE 4.

Sequence variation detected by pyrosequencing of putative isolates of C. rugosa^a

Candida rugosa isolate	Pyrosequencing signature sequence	EMBL match
Position	1                            10                                20                            30
Montero 1	AATAAGATCAA-GAGTCTGTAACAAGCTTAA	C. rugosa, 100% (2 strains)
Montero 2	AATAAGATCgt-GAGTCTGTAACAAGCTTAA	C. rugosa, 100% (9 strains; T)b
NCPF 8690	AATAAGATCAA-GAGTCTGTAACAAGCTTAACTGTTTTA	C. rugosa, 100% (2 strains)
MRL338	AATAAcgTCAAaGgGTCcGTAACAAGCTTAACTGTTTT	C. rugosa, 100% (2 strains)

^aThe pyrosequencing ITS2 signature sequences obtained in the current study (NCPF 8690 and MRL338) are manually aligned against those described by Montero et al. (17), together with the results of BLASTN searches conducted with each signature sequence against the EMBL database (identification returned, percent identity, number of strain matches). The signature sequences for Montero 1 and NCPF 8690 differ only by the additional 8 nucleotides sequenced for NCPF 8690. Hyphens have been introduced to improve alignments; conserved nucleotides are given in uppercase bold letters.

^bT denotes the type strain sequence in this portion of ITS2 (included among the nine strains).