TABLE 1.
Brucella strains of marine mammal origin used in this study
| Species | Strain | Host or source | Latin name of host or source | Geographic origin of strain | MLVA-16 cluster (panel 1 genotype)a | MLSA STb | omp2b-omp2a RFLP patternc | IS711 RFLP patternd (clustere) | IS711 copy no.f | Presence or absence, by fragment-specific PCR, of fragmentg: |
||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| I | II | III | IV | V | ||||||||||
| B. pinnipedialis | 9a-1 | Hooded seal | Cystophora cristata | Norway | C3 (76) | P-I | C | 19 | + | − | − | − | − | |
| 17a-1 | Hooded seal | Cystophora cristata | Norway | C3 (76) | P-I | C | 19 | + | − | − | − | − | ||
| 22a-2 | Hooded seal | Cystophora cristata | Norway | C3 (76) | P-I | C | 19 | + | − | − | − | − | ||
| 23a-1 | Hooded seal | Cystophora cristata | Norway | C3 (75) | P-I | C | 19 | + | − | − | − | − | ||
| 24a-2 | Hooded seal | Cystophora cristata | Norway | C3 (75) | P-I | C | 19 | + | − | − | − | − | ||
| 25a-1 | Hooded seal | Cystophora cristata | Norway | C3 (76) | P-I | C | 19 | + | − | − | − | − | ||
| 30a-1 | Hooded seal | Cystophora cristata | Norway | C3 (74) | P-I | C | 19 | + | − | − | − | − | ||
| 37a-1 | Hooded seal | Cystophora cristata | Norway | C3 (76) | P-I | C | 19 | + | − | − | − | − | ||
| 38g-1 | Hooded seal | Cystophora cristata | Norway | C3 (76) | P-I | C | 19 | + | − | − | − | − | ||
| 39a-1 | Hooded seal | Cystophora cristata | Norway | C3 (76) | P-I | C | 19 | + | − | − | − | − | ||
| 53c-1 | Hooded seal | Cystophora cristata | Norway | C3 (76) | P-I | C | 19 | + | − | − | − | − | ||
| M163/99/10 | Hooded seal | Cystophora cristata | Scotland | C3 (76) | ST25 | P-I | C (4) | 19 | + | − | − | − | − | |
| M603/99/7 | Hooded seal | Cystophora cristata | Scotland | C3 (76) | ST25 | P-I | C (4) | 19 | + | − | − | − | − | |
| M2006/94/6 | Hooded seal | Cystophora cristata | Scotland | C3 (76) | ST25 | P-I | C (4) | 19 | + | − | − | − | − | |
| B2/94 (NCTC 12890; BCCN 94-73h) | Common seal | Phoca vitulina | Scotland | C2 (25) | L-I | D (1) | 23 | + | + | − | − | − | ||
| M13/01/1 | Common seal | Phoca vitulina | Scotland | C2 (25) | ST25 | L-I | D (1) | 23 | + | + | − | − | − | |
| M336/94/1 | Common seal | Phoca vitulina | Scotland | C2 (25) | L-I | D (1) | 23 | + | + | − | − | − | ||
| M339/94/1 | Common seal | Phoca vitulina | Scotland | C2 (25) | L-I | D (1) | 23 | + | + | − | − | − | ||
| M445/99/2 | Common seal | Phoca vitulina | Scotland | C2 (25) | ST25 | L-I | D (1) | 23 | + | + | − | − | − | |
| M514/96/4 | Common seal | Phoca vitulina | Scotland | C2 (25) | ST25 | L-I | D (1) | 23 | + | + | − | − | − | |
| M972/94/1 | Common seal | Phoca vitulina | Scotland | C2 (25) | L-I | D (1) | 23 | + | + | − | − | − | ||
| M621/99/2 | Gray seal | Halichoerus grypus | Scotland | C2 (25) | ST25 | L-I | D (1) | 23 | + | + | − | − | − | |
| M1771/94/1 | Otter | Lutra lutra | Scotland | C2 (25) | ST25 | L-I | D (1) | 23 | + | + | − | − | − | |
| M292/94/1 | Common seal | Phoca vitulina | Scotland | C1 (71) | ST24 | O-I | E (1) | 24 | + | + | − | − | − | |
| M449/02/2 | Common seal | Phoca vitulina | Scotland | C1 (71) | O-I | E (1) | 24 | + | + | − | − | − | ||
| M2466/93/4 | Common seal | Phoca vitulina | Scotland | C2 (72) | L-I | E (1) | 24 | + | + | − | − | − | ||
| M2533/93/1 | Common seal | Phoca vitulina | Scotland | C1 (71) | ST24 | O-I | E (1) | 24 | + | + | − | − | − | |
| M2375/94/3 | Gray seal | Halichoerus grypus | Scotland | C2 (25) | ST25 | L-I | E (1) | 24 | + | + | − | − | − | |
| M194/00/1 | Gray seal | Halichoerus grypus | Scotland | C2 (25) | L-I | E (1) | 24 | + | + | − | − | − | ||
| M192/00/1 | Minke whale | Balaenoptera acutorostrata | Scotland | C1 (71) | ST24 | O-I | E (1) | 24 | + | + | − | − | − | |
| B. ceti | M490/95/1 | Common seal | Phoca vitulina | Scotland | B (23) | ST23 | M-J | F (2) | 30 | + | − | + | + | − |
| 05-0684-1144 | Porpoise | Phocoena phocoena | France | B (23) | M-J | F | 30 | + | − | + | + | − | ||
| M23/03/4 | Porpoise | Phocoena phocoena | Scotland | B (23) | M-J | F (2) | 30 | + | − | + | + | − | ||
| M39/94/1 | Porpoise | Phocoena phocoena | Scotland | B (23) | ST23 | M-J | F (2) | 30 | + | − | + | + | − | |
| M51/04/2 | Porpoise | Phocoena phocoena | Scotland | B (23) | M-J | F (2) | 30 | + | − | + | + | − | ||
| M58/05/1 | Porpoise | Phocoena phocoena | Scotland | B (23) | M-J | F | 30 | + | − | + | + | − | ||
| M78/05/2 | Porpoise | Phocoena phocoena | Scotland | B (23) | ST23 | M-J | F | 30 | + | − | + | + | − | |
| M93/04/3 | Porpoise | Phocoena phocoena | Scotland | B (23) | M-J | F (2) | 30 | + | − | + | + | − | ||
| M117/01/1 | Porpoise | Phocoena phocoena | Scotland | B (23) | M-J | F (2) | 30 | + | − | + | + | − | ||
| M165/03/6 | Porpoise | Phocoena phocoena | Scotland | B (23) | M-J | F (2) | 30 | + | − | + | + | − | ||
| M195/03/10 | Porpoise | Phocoena phocoena | Scotland | B (70) | M-J | F (2) | 30 | + | − | + | + | − | ||
| M234/05/2 | Porpoise | Phocoena phocoena | Scotland | B (23) | ST23 | M-J | F | 30 | + | − | + | + | − | |
| M291/03/2 | Porpoise | Phocoena phocoena | Scotland | B (23) | M-J | F (2) | 30 | + | − | + | + | − | ||
| M499/99/10 | Porpoise | Phocoena phocoena | Scotland | B (23) | M-J | F (2) | 30 | + | − | + | + | − | ||
| M515/96/2 | Porpoise | Phocoena phocoena | Scotland | B (23) | ST23 | M-J | F | 30 | + | − | + | + | − | |
| M615/99/1 | Porpoise | Phocoena phocoena | Scotland | B (23) | M-J | F (2) | 30 | + | − | + | + | − | ||
| M854/98/8 | Porpoise | Phocoena phocoena | Scotland | B (23) | M-J | F (2) | 30 | + | − | + | + | − | ||
| M1570/94/1 | Porpoise | Phocoena phocoena | Scotland | B (23) | ST23 | M-J | F | 30 | + | − | + | + | − | |
| 7763/2 | Bottlenose dolphin | Tursiops truncatus | France | B (23) | ST23 | M-J | F | 30 | + | − | + | + | − | |
| M452/97/2 | Common dolphin | Delphinus delphis | Scotland | B (23) | ST23 | M-J | F (2) | 30 | + | − | + | + | − | |
| M2/00/1 | White-sided dolphin | Lagenorhynchus acutus | Scotland | B (23) | M-J | F (2) | 30 | + | − | + | + | − | ||
| M52/01/1 | White-sided dolphin | Lagenorhynchus acutus | Scotland | B (23) | M-J | F (2) | 30 | + | − | + | + | − | ||
| M181/97/1 | White-sided dolphin | Lagenorhynchus acutus | Scotland | B (23) | ST23 | M-J | F | 30 | + | − | + | + | − | |
| M187/00/1 | White-sided dolphin | Lagenorhynchus acutus | Scotland | B (23) | ST23 | M-J | F (2) | 30 | + | − | + | + | − | |
| M2438/95/1 | White-sided dolphin | Lagenorhynchus acutus | Scotland | B (23) | M-J | F | 30 | + | − | + | + | − | ||
| B202R | Minke whale | Balaenoptera acutorostrata | Norway | B (23) | ST23 | M-J | F (2) | 30 | + | − | + | + | − | |
| B1/94 (NCTC 12891; BCCN 94-74h) | Porpoise | Phocoena phocoena | Scotland | B (23) | M-J | G (2) | 29 | + | − | + | + | − | ||
| M12/00/3 | Porpoise | Phocoena phocoena | Scotland | B (23) | M-J | G (2) | 29 | + | − | + | + | − | ||
| M38/04/3 | Porpoise | Phocoena phocoena | Scotland | B (23) | M-J | G (2) | 29 | + | − | + | + | − | ||
| M103/99/1 | Porpoise | Phocoena phocoena | Scotland | B (23) | M-J | G (2) | 29 | + | − | + | + | − | ||
| M199/04/2 | Porpoise | Phocoena phocoena | Scotland | B (23) | ST23 | M-J | G | 29 | + | − | + | + | − | |
| M1747/98/3 | Porpoise | Phocoena phocoena | Scotland | B (23) | M-J | G (2) | 29 | + | − | + | + | − | ||
| M870/97/1 | White-beaked dolphin | Lagenorhynchus albirostris | Scotland | B (23) | M-J | G | 29 | + | − | + | + | − | ||
| M997/94/2 | White-sided dolphin | Lagenorhynchus acutus | Scotland | B (69) | M-J | G (2) | 29 | + | − | + | + | − | ||
| M2788/97/1 | White-sided dolphin | Lagenorhynchus acutus | Scotland | B (23) | M-J | G (2) | 29 | + | − | + | + | − | ||
| B14/94 | Common dolphin | Delphinus delphis | Scotland | A1 (24) | ST26 | N-K | H (3) | 26 | + | − | − | − | + | |
| M13/05/1 | Striped dolphin | Stenella coeruleoalba | Scotland | A1 (24) | ST26 | N-K | H | 26 | + | − | − | − | + | |
| M40/95/1 | Striped dolphin | Stenella coeruleoalba | Scotland | A1 (24) | ST26 | N-K | H (3) | 26 | + | − | − | − | + | |
| M642/99/2 | Striped dolphin | Stenella coeruleoalba | Scotland | A1 (24) | ST26 | N-K | H (3) | 26 | + | − | − | − | + | |
| M654/99/1 | Striped dolphin | Stenella coeruleoalba | Scotland | A2 (77) | ST26 | N-K | H (3) | 26 | + | − | − | − | + | |
| M656/99/1 | Striped dolphin | Stenella coeruleoalba | Scotland | A1 (24) | ST26 | N-K | H (3) | 26 | + | − | − | − | + | |
| M2194/94/1 | Striped dolphin | Stenella coeruleoalba | Scotland | A1 (24) | ST26 | N-K | H (3) | 26 | + | − | − | − | + | |
| M18/96/1 | White-sided dolphin | Lagenorhynchus acutus | Scotland | A1 (24) | N-K | H | 26 | + | − | − | − | + | ||
| Brucella spp. | 02/611 | Human | Homo sapiens | New Zealand | (78) | ST27 | Q-I | I | 23 | + | − | − | − | − |
a
According to Maquart et al. (17).
b
According to Groussaud et al. (14). ST, sequence type.
d
According to Maquart et al. (18) and results of this study.
e
According to Dawson et al. (8) for the strains indicated.
f
According to Maquart et al. (18) and results of this study.
g
Fragments as numbered in Fig. 1.
h
Reference strains.