TABLE 2.
Primers and adapters used in this study
| Procedure and adapter/primer name | Adapter/primer locationc | Fragment no. | Fragment size (bp) | Nucleotide sequence (5′-3′) |
|---|---|---|---|---|
| Ligation-mediated PCR | ||||
| Eco1 | NAd | NA | NA | GAGTCGTGTTACTCCCATCG |
| Eco2 | NA | NA | NA | AATTCGATGGGAGTAACACG |
| ABg1a | NA | NA | NA | GAGTCGTGTTACTCCCATCG |
| ABg2a | NA | NA | NA | GATCGATGGGAGTAACACGACTC |
| IS1b | IS711 | NA | NA | CGGTTCCATTGCTCATCTGT |
| Fragment-specific PCR | ||||
| I1 | BR1657 | I | 253 | CTACATAGGCATCGCCAGAG |
| II1 | BRA0655 | II | 750 | TGCCATAGGGCACATTGTTC |
| III1 | BR1612 | III | 523 | TTATATGATGCGCCCGGAGG |
| IV1 | BR0613 | IV | 670 | GAAGGTAGAGCCGGAATATC |
| V1 | BR0625 | V | 195 | GCTTTCGCATTGGAGCTTAC |
| IS2 | IS711 | I through V | NA | GCTCACGGCTGTTCTCCTTT |
a
The same adapters and primers were used for LM-PCR with restriction enzymes BglII and BamHI.
b
All LM-PCRs were performed with primer IS1.
c
According to the genome sequence of B. suis 1330 chromosome I or II.
d
NA, not applicable.