TABLE 3.

HBV genotypes and their absolute quantities determined by GQ-PCR

Samplea	Predicted genotype	Genotype determined by the GQ-PCR assay	DNA level (copies/ml) determined by the GQ-PCR assay				Mean DNA level (copies/ml)	SD	CVb (%)
			Run 1	Run 2	Run 3	Run 4
1	B	B	6.981 × 107	6.759 × 107	7.547 × 107	4.087 × 107	6.344 × 107	1.540 × 107	24.284
2	C	C	5.477 × 105	4.958 × 105	9.260 × 105	3.463 × 105	5.790 × 105	2.466 × 105	42.597
3	B	B	2.145 × 105	9.260 × 105	1.255 × 105	3.508 × 105	4.042 × 105	3.560 × 105	89.063
4	B	B	3.021 × 108	6.314 × 108	3.854 × 108	2.541 × 108	3.933 × 108	1.678 × 108	42.664
5	C	C	2.186 × 106	1.193 × 106	9.548 × 105	1.113 × 106	1.362 × 106	0.558 × 106	41.006
6	B	B	1.594 × 109	1.667 × 109	1.906 × 109	1.803 × 109	1.743 × 109	0.139 × 109	7.990
7	B	B	8.282 × 104	6.179 × 104	6.314 × 104	7.954 × 104	7.182 × 104	1.090 × 104	15.179
8	B	B	1.561 × 107	1.479 × 107	1.673 × 107	1.676 × 107	1.597 × 107	0.095 × 107	5.965
9	C	C	2.519 × 106	2.558 × 106	3.580 × 106	4.512 × 106	3.292 × 106	0.950 × 106	28.856
10	B	B	2.222 × 107	1.914 × 107	2.530 × 107	2.720 × 107	2.347 × 107	0.354 × 107	15.082
CS with 6 × 109 copies/ml	B	B	4.636 × 109	6.294 × 109	4.728 × 109	5.107 × 109	5.191 × 109	0.763 × 109	14.696
CS with 3 × 109 copies/ml	C	C	2.595 × 109	2.069 × 109	2.651 × 109	2.844 × 109	2.540 × 109	0.331 × 109	13.051
CS with 6 × 107 copies/ml for genotype B	B and C	B and C	7.362 × 107	7.792 × 107	6.921 × 107	6.660 × 107	7.184 × 107	0.498 × 107	6.937
CS with 3 × 107 copies/ml for genotype C	B and C	B and C	1.351 × 107	1.287 × 107	2.005 × 107	2.290 × 107	1.733 × 107	0.493 × 107	28.442

^aCS, control sample of the known concentration.

^bCV, coefficient of variation.