FIG. 2.

ceacam1a mRNA expression in murine tissues, primary cells, and cell lines. RNAs isolated from 4-week-old C57BL/6 mouse tissues (A), primary cell cultures (B), cell lines (C), 2- to 14-week-old C57BL/6 mouse tissues (D), and C57BL/6 mouse brains (E and F) from mice inoculated i.c. with 50 PFU of rA59 or rJHM.SD were analyzed by qRT-PCR for expression of total ceacam1a mRNA (A to E) or mRNA7 (F). Results are expressed as numbers of cDNA copies of ceacam1a per million cDNA copies of actb (A to D) or as fold changes compared to mock-infected controls (E and F). Error bars represent standard errors of the means (n = 3). Data are representative of two or more independent experiments. LIV, liver; BR, brain; SC, spinal cord; WT, wild type; KO, ceacam1a^−/−; HEP, hepatocyte; MIC, microglia; AST, astrocyte; OLI, oligodendrocyte; CN, cortical neuron; HN, hippocampal neuron.